human nk cell line nk92-mi  (Procell Inc)

Journal: Cancer Cell International

Article Title: Exosomal lncRNA SNHG10 derived from colorectal cancer cells suppresses natural killer cell cytotoxicity by upregulating INHBC

doi: 10.1186/s12935-021-02221-2

Figure Lengend Snippet: EMT exosomal lncRNA SNHG10 inhibited NK cell cytotoxicity. A The efficacy of the overexpression of the lncRNA SNHG10 in SW480 cells was verified by qRT-PCR. B The viability of NK92-MI cells was detected by CCK-8 assay. C The cytotoxicity of NK92-MI cells (pretreated with EMT-exo or not) co-cultured with SW480 cells was detected by LDH assay. D The production of IFN-γ from NK92-MI cells was detected by ELISA. The expression of the toxic molecules perforin and granzyme B in NK92-MI cells (pretreated with EMT-exo or not) co-cultured with SW480 cells was measured by qRT-PCR ( E , F ), western blotting ( H ), and immunofluorescence ( H ). GAPDH was used to normalize gene expression. t -test, * P < 0.05, ** P < 0.01

Article Snippet: The human CRC cell line SW480 (RRID: CVCL_0546) (Procell, CL-0223, China) and the human NK cell line NK92-MI (an interleukin [IL]-2-independent NK cell line) (RRID: CVCL_3755) (Procell, CL-0533, China) were obtained from Procell Life Science Technology.

Techniques: Over Expression, Quantitative RT-PCR, CCK-8 Assay, Cell Culture, Lactate Dehydrogenase Assay, Enzyme-linked Immunosorbent Assay, Expressing, Western Blot, Immunofluorescence, Gene Expression

Journal: Cancer Cell International

Article Title: Exosomal lncRNA SNHG10 derived from colorectal cancer cells suppresses natural killer cell cytotoxicity by upregulating INHBC

doi: 10.1186/s12935-021-02221-2

Figure Lengend Snippet: Exosomal lncRNA SNHG10 regulated the function of NK cells through INHBC. A The proliferation of NK92-MI cells was detected by CCK-8 assay. B The cytotoxicity of NK92-MI cells was detected by LDH assay. C The production of IFN-γ from NK92-MI cells was detected by ELISA. D The expression of the toxic molecules perforin and granzyme B in NK92-MI cells was measured by western blotting. GAPDH was used to normalize gene expression. The data were analyzed by ANOVA followed by Tukey’s test, *NC vs. oe-lncSNHG10 exo, # NC vs. si-INHBC. * P < 0.05, ** P < 0.01, *** P < 0.001, ## P < 0.01, ### P < 0.001

Article Snippet: The human CRC cell line SW480 (RRID: CVCL_0546) (Procell, CL-0223, China) and the human NK cell line NK92-MI (an interleukin [IL]-2-independent NK cell line) (RRID: CVCL_3755) (Procell, CL-0533, China) were obtained from Procell Life Science Technology.

Techniques: CCK-8 Assay, Lactate Dehydrogenase Assay, Enzyme-linked Immunosorbent Assay, Expressing, Western Blot, Gene Expression

Journal: Cancer Cell International

Article Title: Exosomal lncRNA SNHG10 derived from colorectal cancer cells suppresses natural killer cell cytotoxicity by upregulating INHBC

doi: 10.1186/s12935-021-02221-2

Figure Lengend Snippet: Exosomal lncRNA SNHG10 promoted CRC growth by inhibiting NK cells in vivo. A , B Xenograft tumors of BALB/C mice after injection of LV-lncRNA SNHG10-SW480 and LV-vector-SW480 cells (n = 10 per group). C Tumor volume in the two groups. D Tumor weight of each mouse in the two groups. E , F The levels of the INHBC, perforin, and granzyme B proteins in mice were detected by western blotting. Representative images ( G ) and statistical analysis ( H ) of the flow cytometry of NK1.1 in mice in the two groups. I Representative images of IHC of NK1.1 in mice in the two groups. GAPDH was used to normalize gene expression. t -test, * P < 0.05, ** P < 0.01

Article Snippet: The human CRC cell line SW480 (RRID: CVCL_0546) (Procell, CL-0223, China) and the human NK cell line NK92-MI (an interleukin [IL]-2-independent NK cell line) (RRID: CVCL_3755) (Procell, CL-0533, China) were obtained from Procell Life Science Technology.

Techniques: In Vivo, Injection, Plasmid Preparation, Western Blot, Flow Cytometry, Gene Expression